期刊
BMC MICROBIOLOGY
卷 21, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12866-021-02148-8
关键词
Escherichia coli; dsRNA; Capsid protein; Co-expression
类别
资金
- Thailand Graduate Institute of Science and Technology (TGIST)
- National Science and Technology Development Agency (NSTDA)
- Faculty of Science, Mahidol University
Viruses cause significant economic losses to shrimp aquaculture, and the successful construction of a novel E. coli strain capable of co-expressing viral capsid proteins and dsRNA may serve as the basis for cost-effective and efficient production of shrimp antiviral therapeutics in the future.
BackgroundViruses cause significant economic losses to shrimp aquaculture worldwide. In severe cases, they can lead to 100% mortality within a matter of days, hence the aquaculture industry requires antiviral strategies to minimize economic impacts. Currently, a double-stranded RNA (dsRNA)-based platform has been proven effective at a laboratory scale. The bottleneck for its industrialization is the lack of low-cost, efficient and practical delivery approaches. In an effort to bridge the gap between laboratory and farm applications, virus-like particles (VLP) have been used as nanocarriers of dsRNA. However, the implementation of this approach still suffers from high costs and a lengthy procedure, co-expression of subunits of VLP or capsid proteins (CPs) and dsRNA can be the solution for the problem. CP and dsRNA are traditionally expressed in two different E. coli hosts: protease-deficient and RNase III-deficient strains. To condense the manufacturing of dsRNA-containing VLP, this study constructed a novel E. coli strain that is able to co-express viral capsid proteins and dsRNA in the same E. coli cell.ResultsA novel bacterial strain DualX-B15(DE3) was engineered to be both protease- and RNase III-deficiency via P1 phage transduction. The results revealed that it could simultaneously express recombinant proteins and dsRNA.ConclusionCo-expression of viral capsid proteins and dsRNA in the same cell has been shown to be feasible. Not only could this platform serve as a basis for future cost-effective and streamlined production of shrimp antiviral therapeutics, it may be applicable for other applications that requires co-expression of recombinant proteins and dsRNA.
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