期刊
BLOOD
卷 137, 期 18, 页码 2481-2494出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood.2020005627
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资金
- NCMG - MEYS CR [LM2018132]
- Ministry of Health of the Czech Republic [NV18-03-00054]
- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme [802644]
- Czech Science Foundation [20-02566S]
- Ministry of Education, Youth and Sports of the Czech Republic under project CEITEC 2020 [LQ1601]
- Ministry of Education, Youth and Sports of the Czech Republic [MUNI/A/1395/2019]
- European Research Council (ERC) [802644] Funding Source: European Research Council (ERC)
The study revealed changes in miRNA expression in the CLL microenvironment, with members of the miR-29 family playing a crucial role in BCR signaling and prognosis of CLL patients. TRAF4 was identified as a direct target of miR-29, influencing CLL cell responsiveness to CD40 activation and NF-kappa B signaling. The miR-29-TRAF4-CD40 signaling axis modulated by BCR activity demonstrates a novel regulatory mechanism in CLL.
B-cell receptor (BCR) signaling and T-cell interactions play a pivotal role in chronic lymphocytic leukemia (CLL) pathogenesis and disease aggressiveness. CLL cells can use microRNAs (miRNAs) and their targets to modulate microenvironmental interactions in the lymph node niches. To identify miRNA expression changes in the CLL microenvironment, we performed complex profiling of short noncoding RNAs in this context by comparing CXCR4/CD5 intraclonal cell subpopulations (CXCR4(dim)CD5b(right) vs CXCR4(bright)CD5(dim) cells). This identified dozens of differentially expressed miRNAs, including several that have previously been shown to modulate BCR signaling (miR-155, miR-150, and miR-22) but also other candidates for a role in microenvironmental interactions. Notably, all 3 miR-29 family members (miR-29a, miR-29b, miR-29c) were consistently down-modulated in the immune niches, and lower miR-29(a/b/c) levels associated with an increased relative responsiveness of CLL cells to BCR ligation and significantly shorter overall survival of CLL patients. We identified tumor necrosis factor receptor-associated factor 4 (TRAF4) as a novel direct target of miR-29s and revealed that higher TRAF4 levels increase CLL responsiveness to CD40 activation and downstream nuclear factor-kappa B (NF-kappa B) signaling. In CLL, BCR represses miR-29 expression via MYC, allowing for concurrent TRAF4 upregulation and stronger CD40-NF-kappa B signaling. This regulatory loop is disrupted by BCR inhibitors (bruton tyrosine kinase [BTK] inhibitor ibrutinib or phosphatidylinositol 3-kinase [PI3K] inhibitor idelalisib). In summary, we showed for the first time that a miRNA-dependent mechanism acts to activate CD40 signaling/T-cell interactions in a CLL microenvironment and described a novel miR-29-TRAF4-CD40 signaling axis modulated by BCR activity.
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