期刊
BIOTECHNOLOGY JOURNAL
卷 17, 期 3, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.202000449
关键词
aptamers; cell‐ free expression systems; genelet; metabolic engineering; synthetic biology
资金
- National Science Foundation [0832824]
- Defense Advanced Research Projects Agency (DARPA/MTO) Living Foundries program [HR0011-12-C-0065]
- National Research Foundation of Korea - Korean government (MSIT) [NRF-2019R1A2C1086830]
- Direct For Computer & Info Scie & Enginr
- Division of Computing and Communication Foundations [0832824] Funding Source: National Science Foundation
This study designed genetic circuits implementing an RNA aptamer, demonstrating its functionality in a cell-free expression system and in E. coli, showing potential as regulators for synthetic biological circuits and metabolic engineering. The RNA aptamer has the capability of binding to the T7 RNA polymerase and controlling gene expression driven by it, serving as building blocks for logic circuits and transcriptional cascades.
Recent advances in nucleic acids engineering introduced several RNA-based regulatory components for synthetic gene circuits, expanding the toolsets to engineer organisms. In this work, we designed genetic circuits implementing an RNA aptamer previously described to have the capability of binding to the T7 RNA polymerase and inhibiting its activity in vitro. We first demonstrated the utility of the RNA aptamer in combination with programmable synthetic transcription networks in vitro. As a step to quickly assess the feasibility of aptamer functions in vivo, we tested the aptamer and its sequence variants in the cell-free expression system, verifying the aptamer functionality in the cell-free testbed. The expression of aptamer in E. coli demonstrated control over GFP expression driven by T7 RNA polymerase, indicating its ability to serve as building blocks for logic circuits and transcriptional cascades. This work elucidates the potential of T7 RNA polymerase aptamer as regulators for synthetic biological circuits and metabolic engineering.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据