期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 69, 期 3, 页码 1036-1046出版社
WILEY
DOI: 10.1002/bab.2174
关键词
catalytic hairpin assembly; DNA; electrochemistry; immobilization-free; tetraferrocene
资金
- National Natural Science Foundation of China [81860682, 81872968, 81660658]
- JiangXi University of traditional Chinese medicine graduate innovation special fund project [JZYC18S13]
- Chinese medicine graduate innovation special fund project [JZYC18S13]
- Natural Fund of Jiangxi Education Department [GJJ180654]
- Natural Science Foundation of Jiangxi Province [20202BABL206152]
A novel electrochemical DNA sensor utilizing catalytic hairpin assembly (CHA) and tetraferrocene as a signal marker was developed to achieve signal amplification efficiency. The sensor showed good linear correlation and low detection limit under optimal conditions, with excellent selectivity, reproducibility, and stability.
The development of convenient and efficient strategies without using complex nanomaterials or enzymes for signal amplification is very important for bioanalytical applications. Herein, a novel electrochemical DNA sensor was developed by harnessing the signal amplification efficiency of catalytic hairpin assembly (CHA) and a brand-new signal marker tetraferrocene. The prepared sensor had both ends of the probe H2 labeled with tetraferrocene; both ends have a large number of unhybridized T bases, which cause tetraferrocene to move closer to the electrode surface, generating a high-efficiency amplification signal. In the presence of target DNA, it induced strand exchange reactions promoting the formation of double-stranded DNA and recycling of target DNA. Under optimal conditions, the sensor showed a good linear correlation between the peak currents and logarithm of target DNA concentrations (ranging from 0.1 fM to 0.3125 pM) with a detection limit of 0.06 fM, which is obtained by a triple signal-to-noise ratio. Additionally, the prepared sensor possesses excellent selectivity, reproducibility, and stability, demonstrating efficient and stable DNA detection methodology.
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