期刊
BIOSENSORS & BIOELECTRONICS
卷 177, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112952
关键词
Paper microfluidics; Microfluidic chips; Sample preparation; Isothermal amplification; Digital amplification; Commercial products
类别
资金
- National Key R&D Program of China [2018YFC1707700]
- National Natural Science Foundation of China [21904104, 11761161004]
- Key Program for Science and Technology Innovative Research Team in Shaanxi Province of China [2017KCT-22]
- China Postdoctoral Science Foundation [2020M673418]
- Opening Project of Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University [2019LHM-KFKT005, 2019LHM-KFKT006]
- Fundamental Research Funds for the Central Universities [xzy012019067, 1191329125]
Emerging miniaturized and equipment-free nucleic acid testing technologies are driving the development of fully integrated NAT devices at point of care (POC) to simplify operations, reduce contamination risks, and expand applications from qualitative to quantitative and digital detection signals. The review introduces existing commercial products, recent fully integrated microfluidic devices for NAT at POC, and discusses key issues and challenges in scientific research and product development, envisioning the future realization of MARCHED NAT devices.
Benefiting from emerging miniaturized and equipment-free nucleic acid testing (NAT) technologies, fully integrated NAT devices at point of care (POC) with the capability of sample-in-answer-out are proceeding at a break-neck speed to eliminate complex operations and reduce the risk of contamination. Like the development of polymerase chain reaction (PCR) technology (the standard technique for NAT), the detection signal of fully integrated NAT devices has evolved from qualitative to quantitative and recently to digital readout, aiming at expanding their extensive applications through gradually improving detection sensitivity and accuracy. This review firstly introduces the existing commercial products, and then illustrates recent fully integrated microfluidic devices for NAT at POC from the aspect of detection signals (i.e., qualitative, quantitative and digital). Importantly, the key issues of existing commercial products and the main challenges between scientific research and product development are discussed. On this basis, we envision that the MARCHED (miniaturized, automatic, reagent-preloaded, commercializable, high-throughput, environment-independent and disposable) NAT devices are expected to be realized in the near future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据