期刊
BIOSENSORS & BIOELECTRONICS
卷 177, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.112973
关键词
Bacterial detection; Phage recognitions sites; Bacterial receptors; Phage-based biosensors; Fluorescently labelled phages; Recombinant phages; Reporter phages
类别
资金
- National Key Research and Development Program of China [2017YFC1104402]
- China Postdoctoral Science Foundation [2016M602291]
- Chinese Scholarship Council (CSC)
- 3551 Project, Optics Valley of China
Early detection of bacterial pathogens at key interfaces is crucial in minimizing the risk of bacterial diseases. Traditional detection methods are laborious, while molecular diagnostic tools offer more accuracy and sensitivity. Bacteriophages as biorecognition elements have the potential to develop cost-effective, specific, quick, sensitive, and reliable platforms for bacterial pathogen detection.
Some bacterial species are deadly disease-causing pathogens with high morbidity and mortality in humans worldwide. Key interfaces in the transmission of bacterial pathogens include food, water, dairy products, peri-domestic animals, and human interplay. Early-stage detection of such bacteria is crucial in minimizing the risk of bacterial diseases and ensuring early diagnosis. Majority of the conventional microbiological and biochemical detection methods are laborious, require skilled individuals, and are not always accurate. Various molecular diagnostic tools and assays, utilizing sensitive and specific biorecognition elements, such as enzymes, antibodies, and nucleic acids, have been developed and widely used for the detection of pathogenic bacteria. An ideal biorecognition element for the detection of pathogens should be highly specific, stable, sensitive, selective, rapid, easily available, and cost-effective. Bacteriophages, which meet such prerequisites, may be used as biorecognition element alternatives to the currently available molecular probes in the development of cost-effective, specific, quick, sensitive, and reliable platforms (sensors and assays) for the detection of bacterial pathogens. This review details bacteriophage biology and various recognition sites and receptor-binding proteins on the surfaces of tailed phages, which can be used as the recognition sites for specific bacterial detection. It highlights structures and receptors on the surface of bacteria for binding and attachment of specific phages. These features of bacteria and phages provide a basis for establishing methodologies for phage-based bacterial detection, including phage-induced bacterial lysis, phages immobilized on a transducer surface, fluorescently labelled phages, phage-conjugated quantum dots, and recombinant reporter phages, particularly monitored through optical and electrochemical transducer systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据