期刊
BIOSENSORS & BIOELECTRONICS
卷 178, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.112992
关键词
Electrohydrodynamic migration; RNA/DNA concentration; miR-21; Fine needle biopsy
类别
资金
- LAASCNRS micro and nanotechnologies platform, member of the French RENATECH network
- Carnot institute and Ligue contre le Cancer
- Fondation ARC
- ANR [ANR-16-CE18-0028-01, ANR-16-ASTR-0020]
- Agence Nationale de la Recherche (ANR) [ANR-16-CE18-0028, ANR-16-ASTR-0020] Funding Source: Agence Nationale de la Recherche (ANR)
The presented fluorimetry-based technology offers rapid detection of miR-21 concentration using a microfluidic channel, with a reported limit of detection of 2 pM. It shows promising clinical performances for monitoring miR-21 levels in patients with pancreatic cancer.
We present a fluorimetry-based technology for micro-RNA-21 (miR-21) sensing based on the concentration of miR-molecular beacon (MB) complexes and flushing of unbound MB. This concentration module consists of a microfluidic channel with the shape of a funnel operated with electrohydrodynamic actuation. We report a limit of detection of 2 pM in less than 1 min for miR-21 alone, and then demonstrate that miR-21 levels, measured in fine needle biopsy samples, from patients with pancreatic cancer correlate with the reference technique of reverse-transcription polymerase chain reaction (RT-PCR). Altogether, this technology has promising clinical performances for the follow-up of patients with cancer.
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