期刊
BIOSENSORS & BIOELECTRONICS
卷 177, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.112981
关键词
Lame DNA walker; Microsphere; Nicking endonuclease; Biosensor
类别
资金
- National Natural Science Foundation of China [81430054, 81672112, 81802112]
- Chongqing Technology Innovation and Application Demonstration Project [cstc2018jscx-msybX0010]
A novel lame DNA walker that can autonomously move on microsphere-based 3-D tracks is proposed in this study. The walker's motion is driven by cleavage of DNA tracks using a nicking endonuclease, and it enables reaction equilibrium within 30 minutes.
Molecular machines constructed by three-dimensional (3-D) DNA walker have emerged as a hot topic in applications such as novel biosensors, cargo delivery platforms and intracellular imaging. Herein, we first propose a lame DNA walker that can randomly and autonomously move on microsphere-based 3-D track. The stochastic lame walker has a long leg mainly responsible for persistent movement and a short leg cutting substrates rapidly. Its motion is propelled by a nicking endonuclease cleavage of hybridized DNA tracks. Kinetic and persistent study show that the lame DNA walker enables reaction equilibrium at 30 min, need a cleat domain of at least 14 nucleotides and can persistently move on 3-D tracks with an average rate of 6.467 x 10(-11 )M s(-1). We also demonstrate that the lame walker can be used to detect target DNA in the detection range of 10 pM-5 nM with high specificity by toehold exchange mechanism. This work will further expand the performance of 3-D DNA walkers and substantially contributes to the improved understanding of DNA walking systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据