期刊
BIORESOURCE TECHNOLOGY
卷 325, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.124632
关键词
Bacillus megaterium; Enzymatic hydrolysis; Finger millet straw; Polyhydroxybutyrate; Ultrasound-alkaline pretreatment
资金
- Vicerrectoria de Investigacion y Postgrado, Universidad de La Frontera, Chile
- Agencia Nacional de Investigacion y Desarrollo (ANID-Chile) [ANID/FONDAP/15130015]
- Faculty of Science, Chulalongkorn University (CU)
- Graduate School, Chulalongkorn University (CU)
In this study, finger millet straw (FMS) was utilized for the production of Polyhydroxybutyrate (PHB) by Bacillus megaterium strain CAM12. The combination of ultrasound-assisted alkaline pretreatment and enzymatic saccharification of FMS resulted in high delignification and hydrolysis yields, leading to successful PHB production by the strain CAM12. The extracted polymer was confirmed to be PHB through analytical techniques like FTIR, XRD, and NMR, highlighting the potential of FMS hydrolysates as a promising feedstock for PHB production.
In this study, finger millet straw (FMS) was utilized for the production of Polyhydroxybutyrate (PHB) by Bacillus megaterium strain CAM12. Ultrasound-assisted alkaline (NaOH) pretreatment of FMS under optimized conditions followed by enzymatic saccharification resulted in the maximum delignification (72%), hydrolysis yield (84%), glucose yield (86%) and xylose yield (61%). The effects of different pH, temperature, incubation period, inoculum concentration, agitation speed and FMS enzymatic hydmlysates concentration were investigated to improve the PHB production. Under optimized conditions, strain CAM12 used the FMS hydrolysates as the sole carbon source for their growth and produced 8.31 g L-1 of PHB. The extracted polymer on Fourier transform infrared (FTIR), X-ray diffraction (XRD) and Nuclear magnetic resonance (NMR) analyses were confirmed to be PHB. These results suggest the potential of combined ultrasound and alkaline pretreated FMS hydrolysates as a promising feedstock for PHB production.
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