Single-phase and two-phase cultivations using different light regimes to improve production of valuable substances in the anoxygenic photosynthetic bacterium Rhodopseudomonas faecalis PA2

This study aimed to improve biomass, carotenoid, bacteriochlorophyll, protein, lipid, and carbohydrate contents of Rhodopseudomonas faecalis PA2 using different light regimes. Light intensity (4000, 6000, 8000, and 10,000 lx), together with photoperiod (24:0, 16:8, 12:12, and 8:16 h light/dark), was assigned as single-phase (SP) cultivation while two-phase (TP) cultivation used two light intensities (using 4000 lx as the first phase), together with the control of phase shift (3, 6, and 9 days) and photoperiod. Biomass, carotenoid, and bacteriochlorophyll contents were maximized by SP cultivation; light at 8000 lx with light-dark cycle of 24:0 was optimal for pig-ments synthesis. In contrast, TP was useful to enhance storage compounds; protein, lipid, and carbohydrate productivities were significantly increased by 121.69%, 101.69%, and 92.44%, respectively, in TP when compared with SP. This indicates that the novel light strategy proposed in this study was able to manipulate the production of valuable substances in this strain.

Automated summary

This study aimed to improve the production of valuable substances in Rhodopseudomonas faecalis PA2 by using different light regimes. Single-phase cultivation maximized biomass, carotenoid, and bacteriochlorophyll contents, while two-phase cultivation significantly increased protein, lipid, and carbohydrate productivities compared to single-phase cultivation. This novel light strategy was effective in manipulating the production of valuable substances in this strain.