期刊
BIOMATERIALS
卷 271, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2021.120762
关键词
4D hydrogel; Biomimetic corrugation; Electromicrofluidic platform; Biomolecules distribution; Spinal cord injury repair
资金
- Ministry of Science and Technology of Taiwan [MOST 110-2321-B-010006, 109-2221-E-010-004-MY2,108-2221-E-009 -078 -MY3, 108-2321B-010 -008-MY2, 106-2221-E-009 -065 -MY3]
This study developed a hierarchical hybrid gel with NT-3-loaded microcapsules to create an anisotropic structure with specific NT-3 distribution using dielectrophoresis. The material showed dynamic biomimetic microwrinkles and potential applications in soft tissue engineering.
Although traditional 3D scaffolds or biomimetic hydrogels have been used for tissue engineering and regenerative medicine, soft tissue microenvironment usually has a highly anisotropic structure and a dynamically controllable deformation with various biomolecule distribution. In this study, we developed a hierarchical hybrid gelatin methacrylate-microcapsule hydrogel (HGMH) with Neurotrophin-3(NT-3)-loaded PLGA microcapsules to fabricate anisotropic structure with patterned NT-3 distribution (demonstrated as striped and triangular patterns) by dielectrophoresis (DEP). The HGMH provides a dynamic biomimetic sinuate-microwrinkles change with NT-3 spatial gradient and 2-stage time-dependent distribution, which was further simulated using a 3D finite element model. As demonstrated, in comparison with striped-patterned hydrogel, the triangular-patterned HGMH with highly anisotropic array of microcapsules exhibits remarkably spatial NT-3 gradient distributions that can not only guide neural stem cells (NSCs) migration but also facilitate spinal cord injury regeneration. This approach to construct hierarchical 4D hydrogel system via an electromicrofluidic platform demonstrates the potential for building various biomimetic soft scaffolds in vitro tailed to real soft tissues.
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