期刊
EPIGENETICS
卷 11, 期 6, 页码 438-448出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15592294.2016.1176816
关键词
Blood miRNA; CVD; HDL; microRNA; TFA
资金
- Reseau de recherche en sante cardiometabolique, diabete et obesite (CMDO) of the Fonds de recherche du Quebec en sante (FRQS)
- Etienne-Le Bel Clinical research center
- ECOGENE-21 Clinical Research Center
- Faculte de medecine et des sciences de la sante (FMSS) of Universite de Sherbrooke
- FRQS
- Canadian Institutes of Health Research (CIHR)
- Dairy Farmers of Canada
- Novalait Inc.
- Natural Sciences and Engineering Research Council of Canada
A high consumption of trans fatty acids (TFAs) is associated with an increased risk of cardiovascular diseases (CVDs). High-density lipoproteins (HDLs) have many cardioprotective properties and transport functional microRNAs (miRNAs) to recipient cells. We hypothesized that dietary TFAs modify the HDL-carried miRNA profile, therefore modulating its cardioprotective properties. We assessed whether consumption of dietary TFAs modifies HDL-carried miR-223-3p and miR-135a-3p concentration and the inter-relationship between diet-induced changes in HDL-carried miRNA concentration and CVD risk markers. In a double blind, randomized, crossover, controlled study, 9 men were fed each of 3 experimental isoenergetic diets: 1) High in industrial TFA (iTFA; 3.7% energy); 2) High in TFA from ruminants (rTFA; 3.7% energy); 3) Low in TFA (control; 0.8% energy) for 4 weeks each. HDLs were isolated by ultracentrifugation and miRNAs were quantified by RT-qPCR. Variations in HDL-miR-223-3p concentration were negatively correlated with variations in HDL-cholesterol after the iTFA diet (r(s)=0.82; P=0.007), and positively correlated with variations in C-reactive protein concentration after the rTFA diet (r(s)=0.75; P=0.020). Variations in HDL-miR-135a-3p concentration were positively correlated with variations in total triglyceride (TG) concentration following the iTFA diet (r(s)=0.82; P=0.007), and with variations in low-density lipoprotein (LDL)-TG concentration following the rTFA diet (r(s)=0.83; P=0.005), compared to the control diet. However, the consumption of dietary TFAs has no significant unidirectional impact on HDL-carried miR-223-3p and miR-135a-3p concentrations. Our results suggest that the variability in the HDL-carried miRNAs response to TFA intake, by being associated with variations in CVD risk factors, might reflect physiological changes in HDL functions.
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