期刊
BIOINORGANIC CHEMISTRY AND APPLICATIONS
卷 2021, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2021/9920890
关键词
-
资金
- Fundamental Research Grant Scheme (FRGS) [FRGS/1/2018/STG07/USM/02/9]
- Ministry of Higher Education, Malaysia [203.CIPPT.6711684]
- USM Fellowship Scheme
The biosynthesized silver nanoparticles using extracts from Clinacanthus nutans demonstrated promising potential in antioxidant, antimicrobial, and cytotoxic activities, showing high inhibition against Staphylococcus aureus, good antioxidant activities, and cytotoxic effects on cancer cells.
Silver nanoparticles (AgNPs) previously synthesised using leaf (AgNP-L) and stem (AgNP-S) extracts of Clinacanthus nutans (C. nutans) were tested to evaluate antimicrobial, antioxidant, and cytotoxicity activities. The AgNPs showed good inhibition against bacteria, but not fungi. The inhibition results showed the highest activity against Staphylococcus aureus (S. aureus) with 11.35 mm (AgNP-L) and 11.52 mm (AgNP-S), while the lowest inhibition was against Escherichia coli (E. coli) with 9.22 mm (AgNP-L) and 9.25 mm (AgNP-S) in the disc diffusion method. The same trend of results was noted in the well diffusion method. The IC50 of AgNP-L and AgNP-S in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2 '-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays was 417.05 mu g/mL and 434.60 mu g/mL, as well as 304.31 mu g/mL and 326.83 mu g/mL, respectively. Ferric reducing power (FRAP) assay showed that AgNP-L [872.389 mu mol/L Fe(II)] and AgNP-S [612.770 mu mol/L Fe(II)] exhibited significantly (p < 0.05) greater antioxidant activities than leaf extract (CNL) [152.260 mu mol/L Fe(II)] and stem extract (CNS) [110.445 mu mol/L Fe(II)] of C. nutans. The AgNPs were also proven to possess cytotoxic effects on the breast (MCF-7), cervical (HeLa), and colon (HT-29) cancer cells in a dose-dependent manner. AgNP-S and AgNP-L showed significantly (p < 0.05) higher cytotoxicity against MCF-7 (117.43 mu g/mL) and HT-29 (78.47 mu g/mL), respectively. In conclusion, the biosynthesised AgNPs from aqueous extract leaves and stem of C. nutans have demonstrated promising potential towards antioxidant, antimicrobial, and cytotoxicity activities.
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