期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
卷 1868, 期 5, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbamcr.2021.118972
关键词
Cell mechanics; Collagen hydrogel; Nano-indentation; Force-displacement measurement; TGF-beta 1; Finite element
资金
- Ageing Research at King's (ARK)
The study utilized nano-biomechanical technique and mathematical modeling to measure the contractility of dermal fibroblasts from donors of different ages. It was found that fibroblasts from aged donors exhibited greater basal contraction force, while those from young donors showed significantly larger contractile force in response to TGF-beta 1 treatment. These findings provide new insights for regenerative medicine and as a biomarker of dermal ageing processes.
Dermal fibroblasts play a key role in maintaining homoeostasis and functionality of the skin. Their contractility plays a role in changes observed during ageing, especially in processes such as wound healing, inflammation, wrinkling and scar tissue formation as well as structural changes on extracellular matrix. Although alternations in skin physiology and morphology have been previously described, there remains a paucity of information about the influence of chronological ageing on dermal fibroblast contractility. In this study, we applied a novel nano-biomechanical technique on cell-embedded collagen hydrogels in combination with mathematical modelling and numerical simulation to measure contraction forces of normal human dermal fibroblasts (NHDF). We achieved quantitative differentiation of the contractility of cells derived from 'young' (< 30 years old) and 'aged' (> 60 years old) donors. Transforming growth factor beta 1 (TGF-beta 1) was used to stimulate the fibroblasts to assess their contractile potential. NHDF from aged donors exhibited a greater basal contractile force, while in contrast, NHDF from young donors have shown a significantly larger contractile force in response to TGF-beta 1 treatment. These findings validate our nano-biomechanical measurement technique and provide new insights for considering NHDF contractility in regenerative medicine and as a biomarker of dermal ageing processes.
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