期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
卷 1868, 期 8, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbamcr.2021.119060
关键词
Brain tumors; Glioma; Glioblastoma; Store-operated Ca2+ entry; Purinergic signalling
资金
- Ministry of Science and Innovation of Spain [RTI2018-099298-B-100]
- Junta de Castilla y Leon, Spain [VA294P18]
- IBGM Strategic Program grant from Junta de Castilla y Leon, Spain [CCVC8485]
- European Social Fund
- Spanish Association Against Cancer (AECC)
Brain tumors consist of a variety of tumor types with different levels of malignancy. The study found that malignant tumor cells exhibit calcium responses to ATP and have significantly increased store-operated Ca2+ entry. Furthermore, high grade gliomas have larger SOCE compared to low grade gliomas, suggesting a correlation between SOCE and glioma progression.
Brain tumors comprise a large series of tumor cancer from benign to highly malignant gliomas and metastases from primary tumors outside the brain. Intracellular Ca2+ homeostasis is involved in a large series of cell functions including cell proliferation, migration, and cell death. Store-operated Ca2+ entry (SOCE), the most important Ca2+ entry pathway in non-excitable cells, is involved in cell proliferation and migration and enhanced in tumor cells from breast cancer, colon cancer and cell lines derived from glioblastoma but there are almost no studies in human primary glioblastoma cells or other brain tumors. We have developed a single procedure to obtain primary cells from a large series (n = 49) of human brain tumors including schwannomas, meningiomas, oligodendrogliomas, astrocytomas, glioblastomas and brain metastases from ovary, breast and lung. Cells were characterized by immunofluorescence and subjected to Ca2+ imaging to investigate resting intracellular Ca2+ levels, Ca2+ responses to physiological agonists as well as voltage-operated Ca2+ entry and SOCE. We found significant differences in resting intracellular Ca2+ and Ca2+ responses to plasma membrane depolarization and ATP among the different tumor cells. Only malignant tumor cells, displayed Ca2+ responses to ATP. SOCE is significantly increased in malignant gliomas whereas voltage-gated Ca2+ entry is decreased. In addition, SOCE is significantly larger in high grade gliomas than in low grade gliomas suggesting that SOCE increases with glioma progression. These data may provide new insights on the role of intracellular Ca2+ and purinergic signalling in brain tumors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据