4.6 Article

Cobalt chloride-simulated hypoxia elongates primary cilia in immortalized human retina pigment epithelial-1 cells

期刊

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2021.03.097

关键词

Primary ciliogenesis; Cilia length; Cobalt chloride; Hypoxia; Rabaptin-5; Endocytosis

资金

  1. National Natural Science Foundation of China [31601146, 81971303]
  2. National-Guangdong Joint Engineering Laboratory for Diagnosis and Treatment of Vascular Diseases and Guangdong Provincial Engineering and Technology Center for Diagnosis and Treatment of Vascular Diseases
  3. National Natural Science Foundation of China-Key Program [91649203]
  4. National Key R&D Program of China [2016YFE0204700, 2017YFA0103302]

向作者/读者索取更多资源

Hypoxia may elongate primary cilia by downregulating Rabaptin-5 involved in endocytosis. The coordination between endocytosis and ciliogenesis can be utilized by cells to adapt to hypoxia.
Primary cilia are microtubule-based organelles that are involved in sensing micro-environmental cues and regulating cellular homeostasis via triggering signaling cascades. Hypoxia is one of the most common environmental stresses that organ and tissue cells may often encounter during embryogenesis, cell differentiation, infection, inflammation, injury, cerebral and cardiac ischemia, or tumorigenesis. Although hypoxia has been reported to promote or inhibit primary ciliogenesis in different tissues or cultured cell lines, the role of hypoxia in ciliogenesis is controversial and still unclear. Here we investigated the primary cilia change under cobalt chloride (CoCl2)-simulated hypoxia in immortalized human retina pigment epithelial-1 (hTERT RPE-1) cells. We found CoCl2 treatment elongated primary cilia in a time- and dose-dependent manner. The prolonged cilia recovered back to near normal length when CoCl2 was washed out from the cell culture medium. Under CoCl2-simulated hypoxia, the protein expression levels of HIF-1/2 alpha and acetylated-alpha-tubulin (cilia marker) were increased, while the protein expression level of Rabaptin-5 is decreased during hypoxia. Taken together, our results suggest that hypoxia may elongate primary cilia by downregulating Rabaptin-5 involved endocytosis. The coordination between endocytosis and ciliogenesis may be utilized by cells to adapt to hypoxia. (C) 2021 Elsevier Inc. All rights reserved.

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