期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 552, 期 -, 页码 78-83出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2021.03.034
关键词
Embryonic stem cells; Pluripotency; Differentiation; p53; p53(-/-); Autophagy; Ulk1
资金
- Russian Foundation for Basic Research [18-015-00230]
Autophagy plays a critical role in early embryonic development, particularly in blastocyst formation. The absence of p53 does not affect autophagy activation in pluripotent cells but hinders differentiation induced by retinoic acid. Despite p53 deletion, Ulk1-dependent autophagy is still activated in response to serum deprivation in mESCs, but impaired during differentiation induced by retinoic acid.
Autophagy is known to play a critical role in the early stages of embryogenesis including the formation of blastocyst. The existence of p53 protein-deficient mice may identify that p53 is not indispensable for the activation of autophagy in pluripotent cells derived from the inner cell mass of the blastocyst. We utilized a p53-knockout (KO) mouse embryonic stem cell (mESC) line to investigate the contribution of p53 in autophagy. We showed that lack of p53 has no effect on cell pluripotency but significantly hinders the differentiation process induced by retinoic acid. Using MRT68921, we revealed that Ulk1-dependent autophagy is activated in response to serum deprivation despite the deletion of p53 in mESCs. However, under retinoic acid-induced differentiation, the accumulation of autophagosomes and lysosomes is impaired in p53 KO mESCs, indicating a critical role of p53 in the regulation of autophagy upon differentiation. (C) 2021 Elsevier Inc. All rights reserved.
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