期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 552, 期 -, 页码 73-77出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2021.03.043
关键词
Autotransporter; beta-barrel assembly machinery; Outer membrane protein; TtOmp85
资金
- National Natural Science Foundation of China [31770901]
- CAMS Innovation Fund for Medical Sciences [2017-I2M-1-012]
- Fundamental Research Funds for the Central Universities [3332019065]
- State Key Laboratory Special Fund [2060204]
- Shandong Provincial Natural Science Foundation [ZR2016CM32]
- Deutsche Forschungsgemeinschaft [FA 1278/1-1]
Research shows that a single TtOmp85 protein can replace the collective function of the five subunits constituting the E. coli BAM, providing new insights into the search for the primitive form of a functional BAM.
The biogenesis of outer membrane proteins requires the function of beta-barrel assembly machinery (BAM), whose function is highly conserved while its composition is variable. The Escherichia coli BAM is composed of five subunits, while Thermus thermophilus seems to contain a single BAM protein, named TtOmp85. To search for the primitive form of a functional BAM, we investigated and compared the function of TtOmp85 and E. coli BAM by use of a reconstitution assay that examines the integration of OmpA and BamA from E. coli and TtoA from T. thermophilus, as well as the translocation of the E. coli Ag43. Our results show that a single TtOmp85 protein can substitute for the collective function of the five subunits constituting E. coli BAM. (C) 2021 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据