MRNIP is essential for meiotic progression and spermatogenesis in mice

Meiotic homologous recombination (HR) initiates with the programmed generation of DNA double strand breaks (DSBs), which result in the exchange of genetic information and genome diversity. This process requires the tight cooperation of the MRE11-RAD50-NBS1 (MRN) complex to promote DSB formation and DNA end resection. However, the mechanism regulating MRN complex remains to be explored. In the present study, we report that MRN-interacting protein, MRNIP, is a novel factor for HR and is crucial for the expression of the MRN complex and loading of recombinases DMC1/RAD51. Knockout of Mrnip in mice led to aberrant synapsis, impaired HR, and male subfertility. In conclusion, MRNIP is a novel HR factor that probably promotes meiotic progression through the MRN complex. ? 2021 Elsevier Inc. All rights reserved.

Automated summary

Given the importance of the MRN complex in HR, this study identifies MRNIP as a novel HR factor crucial for the expression of the MRN complex and loading of recombinases, thus promoting meiotic progression.