期刊
AUTOPHAGY
卷 17, 期 9, 页码 2576-2585出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15548627.2021.1911549
关键词
ATG14; autophagy; autophagic vacuolar myopathy; cardiomyopathy; inclusion body myopathy; RB1CC1; RNA-binding protein; ULK1; ULK2
类别
资金
- American Lebanese Syrian Associated Charities
- National Institutes of Health [R01 GM132231, R01 MH115058]
- Robert Packard Center for ALS Research, Johns Hopkins University
Defects in macroautophagy/autophagy are implicated in the pathogenesis of neuromuscular and heart diseases. The study generated muscle-specific rb1cc1- and atg14-conditional knockout (cKO) mice and found that atg14-cKO mice developed hypertrophic cardiomyopathy, while rb1cc1-cKO mice showed features of autophagic vacuolar myopathy. Tissue-specific differences between skeletal and cardiac muscles in their reliance on core autophagy proteins and unique roles for ULK1-ULK2 and RB1CC1 were highlighted in the development of TARDBP(+) pathology.
Defects in macroautophagy/autophagy are implicated in the pathogenesis of neuromuscular and heart diseases. To precisely define the roles of autophagy-related genes in skeletal and cardiac muscles, we generated muscle-specific rb1cc1- and atg14-conditional knockout (cKO) mice by using Ckm/Ckmm2-Cre and compared their phenotypes to those of ulk1 ulk2-conditional double-knockout (cDKO) mice. atg14-cKO mice developed hypertrophic cardiomyopathy, which was associated with abnormal accumulation of autophagic cargoes in the heart and early mortality. Skeletal muscles of both atg14-cKO and rb1cc1-cKO mice showed features of autophagic vacuolar myopathy with ubiquitin(+) SQSTM1(+) deposits, but only those of rb1cc1-cKO mice showed TARDBP/TDP-43(+) pathology and other features of the inclusion body myopathy-like disease we previously described in ulk1 ulk2-cDKO mice. Herein, we highlight tissue-specific differences between skeletal and cardiac muscles in their reliance on core autophagy proteins and unique roles for ULK1-ULK2 and RB1CC1 among these proteins in the development of TARDBP(+) pathology.
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