期刊
ARCHIVES OF VIROLOGY
卷 166, 期 6, 页码 1775-1778出版社
SPRINGER WIEN
DOI: 10.1007/s00705-021-05041-x
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资金
- National Key Research and Development Program of China [2017YFD0101803]
- Bulk Vegetable Industry Technology Systems of China [CARS-23-G-26]
In this study, a novel partitivirus named BCCV1 was discovered in Brassica campestris L. ssp. chinensis, with its full genome sequence assembled using NGS and adapter-ligation-mediated amplification. BCCV1 contains two dsRNA segments encoding RdRp and CP, and phylogenetic analysis placed it as a new member of the genus Deltapartitivirus, family Partitiviridae. This is the first report of a member of the family Partitiviridae identified in Brassica campestris L. ssp. chinensis.
In the present work, we report the discovery and complete genome sequence of a novel partitivirus identified from Brassica campestris L. ssp. chinensis, which we have named Brassica campestris chinensis cryptic virus 1 (BCCV1). Next-generation sequencing (NGS) combined with adapter-ligation-mediated amplification allowed assembly of the full-length genome sequence of BCCV1. The genome of BCCV1 contains two dsRNA segments, dsRNA1 (1595 bp) and dsRNA2 (1591 bp), which encode a conserved RNA-dependent RNA polymerase (RdRp) and a putative capsid protein (CP), respectively. Homology searches and phylogenetic analysis of the 479-aa RdRp and 438-aa CP showed that BCCV1 is a new member of the genus Deltapartitivirus, family Partitiviridae. This is the first report of the identification of a member of the family Partitiviridae in Brassica campestris L. ssp. chinensis.
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