期刊
ARCHIVES OF PHARMACAL RESEARCH
卷 44, 期 5, 页码 514-524出版社
PHARMACEUTICAL SOC KOREA
DOI: 10.1007/s12272-021-01329-3
关键词
Ginkgo biloba; MS; MS; CFM-ID 3; 0; ECD calculations; Alkaline phosphatase
资金
- National Research Foundation of Korea (NRF) - Korean government [2019R1A5A2027340, 2021R1A2C2007937]
- Korea Institute of Science and Technology intramural research Grant [2E30641]
- Nano Convergence Industrial Strategic Technology Development Program - Ministry of Trade, Industry & Energy (MOTIE, Korea) [20000105]
- Korea Evaluation Institute of Industrial Technology (KEIT) [20000105] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2E30641, 2021R1A2C2007937, 2019R1A5A2027340] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ginkgo biloba, known as a living fossil, has been historically used in traditional medicine and food. Two new coumaric acid-aliphatic alcohol hybrids, ginkwanghols A and B, isolated from G. biloba leaves, show the potential to promote osteogenic differentiation in mouse mesenchymal stem cells.
Ginkgo biloba (Ginkgoaceae), commonly known as ginkgo, is called a living fossil, and it has been cultivated early in human history for various uses in traditional medicine and as a source of food. As part of ongoing research to explore the chemical diversity and biologically active compounds from natural resources, two new coumaric acid-aliphatic alcohol hybrids, ginkwanghols A (1) and B (2) were isolated from the leaves of G. biloba. The coumaric acid-aliphatic alcohol hybrids of natural products have rarely been reported. The structures of the new compounds were determined by extensive NMR spectroscopic analysis, HRESI-MS, and quantum chemical ECD calculations, and by comparing the experimental HRESI-MS/MS spectrum of chemically transformed compound 1a with the predicted HRESI-MS/MS spectra proposed from CFM-ID 3.0, a software tool for MS/MS spectral prediction and MS-based compound identification. Ginkwanghols A (1) and B (2) increased alkaline phosphatase (ALP) production in C3H10T1/2, a mouse mesenchymal stem cell line, in a dose-dependent manner. In addition, ginkwanghols A and B mediated the promotion of osteogenic differentiation as indicated by the induction of the mRNA expression of the osteogenic markers ALP and osteopontin (OPN).
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