期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 91, 期 -, 页码 52-58出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2016.05.011
关键词
Feruloyl esterase; Enzymatic hydrolysis; Corn cob; Vine trimming shoot; Ferulic acid
资金
- Spanish Ministry of Economy and Competitiveness - FEDER funds of the European Union [CTQ2015-71436-C2-1-R]
- Spanish Ministry of Education, Culture and Sports
Ferulic acid esterases (FAE) were produced by Aspergillus terreus CECT 2808 from vine trimming shoots (VTS) and corn cob. Later, the fungal extracts thus obtained were used to enzymatically release ferulic acid (FA) from both substrates. Our findings showed a higher FAE activity in the enzymatic extracts produced on corn cob (0.070 +/- 0.004 U/mL). Nevertheless, the enzymatic extracts produced on VTS demonstrated a better performance for FA release from both corn cob (2.05 +/- 0.01 mg/g) and VTS (0.19 +/- 0.003 mg/g). This result was probably because of the higher xylanase/FAE ratio determined in VTS extract. Therefore, an additional assay was carried out by supplementing corn cob extract with a commercial xylanase to test the influence of FAE/xylanase ratio in FA release. The results revealed the relevance of the FAE/xylanase ratio for an optimal FA release. (C) 2016 Elsevier Inc. All rights reserved.
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