期刊
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
卷 106, 期 4, 页码 -出版社
WILEY
DOI: 10.1002/arch.21783
关键词
Bombyx mori; l‐ ascorbic acid (AsA); l‐ gulose biosynthesis pathway; l‐ gulonolactone oxidase (GULO)
资金
- National Natural Science Foundation of China [31972625, 31672492]
- China Agriculture Research System (CARS) [CARS-18]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX20_2671]
The study found that silkworms can synthesize Vitamin C through a specific pathway at certain developmental stages, but the synthesis ability varies with different developmental stages.
Vitamin C (VC) is an essential nutrient for many animals. However, whether insects, including Bombyx mori, can synthesize VC remains unclear. In this article, the optimized HPLC method was used to determine the content of l-ascorbic acid (AsA) in silkworm eggs, larvae and pupae, and the activity of l-gulono-1,4-lactone oxidase (GULO), a key enzyme in VC synthesis. The RNA interference method was used to determine the effect of the BmGulo-like gene on embryonic development and GULO activity in the pupal fat body. The AsA content increased significantly during E144 h-E168 h in the late embryonic stage and P48 h-P144 h in the middle-late pupal stage, in which exogenous VC was not ingested. Furthermore, the body AsA content in larvae fed VC-free feed also increased with larval stage. The GULO enzymatic activity was present in eggs and the fat bodies of larvae and pupae, even when the larvae were reared with fresh mulberry leaves. Moreover, the activity was higher in the later embryonic stages (E144 h-E168 h) and the early pupal stage (before P24 h). The GULO activity in the pupal fat body dramatically decreased when the screened BmGulo-like gene (BGIBMGA005735) was knocked down with small interfering RNA; in addition, the survival rate and hatching rate of eggs significantly decreased 21% and 44%, respectively, and embryonic development was delayed. Thus, Bombyx mori can synthesize AsA through the l-gulose pathway, albeit with low activity, and this synthesis ability varies with developmental stages.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据