期刊
AQUATIC TOXICOLOGY
卷 233, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2021.105790
关键词
Tetrahymena; Titanate nanotubes; Cytotoxicity; Uptake; Excretion
资金
- National Key R&D Program of China [2017YFF0211203]
- National Natural Science Foundation of China [41701541]
The study revealed that exposure to different concentrations of TNTs had varying effects on cell density and cytotoxicity in Tetrahymena, with mutant strains NP1 and SB255 taking up greater or comparable amounts of TNTs in a short period of time than the wild type strain SB210, while prolonged exposure to high concentrations of TNTs resulted in significant decreases in cell density.
The potential exposure of titanate nanotubes (TNTs) to wildlife and humans may occur as a result of increased use and application as functional nanomaterials. However, there is a dearth of knowledge regarding the pathways of uptake and excretion of TNTs and their toxicity in cells. In this study, three strains of the Tetrahymena genus of free-living ciliates, including a wild type strain (SB210) and two mutant strains (SB255: mucocyst-deficient; NP1: temperature-sensitive mouthless), were used to study the pathways of uptake and excretion and evaluate the cytotoxicity of TNTs. The three Tetrahymena strains were separately exposed to 0, 0.01, 0.1, 1 or 10 mg/L of TNTs, and cells were collected at different time points for quantification of intracellular TNTs (e.g., 5, 10, 20, 40, 60, 90 and 120 min) and evaluation of cytotoxicity (12 and 24 h). TNT contents in NP1 and SB255 were greater or comparable to the contents in SB210 while exposure to 10 mg/L TNTs in 120 min. Furthermore, exposure to 10 mg/L TNTs for 24 h caused greater decreases in cell density of NP1 (38.2 %) and SB255 (36.8 %) compared with SB210 (26.5 %) and upregulated the expression of caspase 15 in SB210. Taken together, our results suggested that TNT uptake by pinocytosis and excretion by exocytosis in Tetrahymena, and the exposure could cause cytotoxicity which can offer novel insights into the accumulation kinetics of nanotubes and even nanomaterials in single cell.
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