期刊
APPLIED SURFACE SCIENCE
卷 546, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.apsusc.2021.148984
关键词
Biomolecular interactions; Tris buffer; Surface plasmon resonance; Biosensor
类别
资金
- Czech Science Foundation [20-23787X, 19-02739S]
Functional materials with organic coatings on inorganic substrates are considered potential platforms for various applications, leading to the investigation of their interactions with the micro-environment becoming a key research direction in surface science. This study focused on the interactions of Tris buffer with self-assembled monolayers (SAMs) of alkanethiols and short DNAs using a surface plasmon resonance (SPR) biosensor, showing that the interaction is a complex multiphasic process. The study also revealed how the interaction of Tris with immobilized DNA molecules can affect the determination of surface concentrations and hybridization efficiencies.
Functional materials employing organic coatings on inorganic substrates are perceived as potential platforms for applications in a variety of fields. Therefore, the investigation of interactions of such systems with the micro-environment has become an important research direction in surface science. Herein, we study the interaction of one of the buffers most commonly used in biological studies, Tris buffer, with self-assembled monolayers (SAMs) of alkanethiols and short DNAs using a surface plasmon resonance (SPR) biosensor. We show that the interaction between Tris and carboxylic groups of SAMs is a complex multiphasic process. We demonstrate that Tris base binds to the protonated carboxylic groups. When those groups become deprotonated, Tris base dissociates and Tris acid is attracted, which results in the formation of a diffuse layer over the charged surface. In addition, we show that the interaction of Tris with the immobilized DNA molecules biases the determination of surface concentrations of the immobilized DNA molecules and thus also the determination of hybridization efficiencies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据