期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 44, 期 -, 页码 53-61出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.etap.2016.02.009
关键词
Cordyceps cicadae; Adenosine; Glutamate; Neuroprotection; PC12 cells; Oxidative toxicity
资金
- National Natural Science Foundation of China [81072985, 81373480, 81573529]
Glutamate has been proven to induce oxidative stress through the formation of reactive oxygen species (ROS) and increased calcium overload which results in neuronal injury, development of neurodegenerative diseases and death. Adenosine is one of the bioactive nucleosides found in Cordyceps cicadae and it has displayed several pharmacological activities including neuroprotection. In this study, the protective effects of adenosine from C. cicadae against glutamate-induce oxidative stress in PC12 cells were evaluated. The exposure of PC12 cells to glutamate (5 mM) induced the formation of ROS, increased Ca2+ influx, endoplasmic reticulum (ER) stress and up regulated the expression of pro-apoptotic factor Bax. However, pretreatment with adenosine markedly increased cell viability, decreased the elevated levels of ROS and Ca2+ induced by glutamate. Furthermore adenosine increased the activities of GSH-Px and SOD, as well as retained mitochondria membrane potential (MMP), increased Bcl-2/Bax ratio, and reduced the expression of ERK, p38, and JNK. Overall, our results suggest that adenosine may be a promising potential therapeutic agent for the prevention and treatment of neurodegenerative disorders. (C) 2016 Elsevier B.V. All rights reserved.
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