期刊
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
卷 114, 期 6, 页码 719-730出版社
SPRINGER
DOI: 10.1007/s10482-021-01552-4
关键词
Crudilactis; Pseudomonas; Raw milk; Taxonomy; Whole genome sequence
类别
资金
- Organisation de Defense et de Gestion des AOP Laitieres de Normandie
- FWO Vlaanderen [1S64720N]
- EOS [30650620]
Strains belonging to the Pseudomonas genus, including UCMA 17988 isolated from raw milk, have been found to form a novel species named Pseudomonas crudilactis. This strain was reported to produce antimicrobial lipopeptides and differed significantly from its closest relatives based on DNA hybridization and nucleotide identity values.
Strains belonging to the Pseudomonas genus have been isolated worldwide from various biotic (humans, animals and plant tissues) and abiotic (food, soil, water and air) environments. Raw milk provides a favorable environment for the growth of a broad spectrum of microorganisms, including Pseudomonas. Here we present the description of Pseudomonas sp. UCMA 17988 isolated from raw milk, which was previously reported to produce new antimicrobial lipopeptides. MultiLocus Sequence Analysis of four housekeeping genes (16S rRNA, gyrB, rpoD and rpoB), whole genome sequence comparison (orthoANI value, original ANI value and dDDH value), microscopy, FAME analysis, and biochemical tests were performed. Digital DNA-DNA hybridization and average nucleotide identity values between strain UCMA 17988 and its closest relatives, P. helmanticensis CECT 8548(T) (46.9%, 92.07%) and P. baetica CECT 7720(T) (26.8%, 88.50%), rate well below the designed threshold for assigning prokaryotic strains to the same species. In conclusion, strain UCMA 17988 belongs to a novel species, for which the name Pseudomonas crudilactis sp. nov (type strain UCMA 17988(T) = DSM 109949(T) = LMG 31804(T)) is proposed.
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