In Situ Programming of CAR T Cells

Gene therapy makes it possible to engineer chimeric antigen receptors (CARs) to create T cells that target specific diseases. However, current approaches require elaborate and expensive protocols to manufacture engineered T cells ex vivo, putting this therapy beyond the reach of many patients who might benefit. A solution could be to program T cells in vivo. Here, we evaluate the clinical need for in situ CAR T cell programming, compare competing technologies, review current progress, and provide a perspective on the long-term impact of this emerging and rapidly flourishing biotechnology field.

Automated summary

Gene therapy enables the creation of CAR-T cells to target specific diseases, but current manufacturing protocols are costly and complex, limiting access for many patients. In vivo programming of T cells could potentially overcome these challenges and expand the reach of this therapy.