期刊
ENVIRONMENTAL TOXICOLOGY
卷 32, 期 4, 页码 1191-1201出版社
WILEY
DOI: 10.1002/tox.22316
关键词
uterus; glyphosate-based herbicide; estrogen receptor; progesterone receptor
资金
- Consejo Nacional de Investigaciones Cientificas y Tecnicas [PIP 112-200801-00218]
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT 2011-1491, PICT 2014-1522]
- Universidad Nacional del Litoral [C.A.I. + D. 2011 501 201101 00423 LI, PAITI 2011]
Glyphosate is the active ingredient of several herbicide formulations. Different reports suggest that glyphosate-based herbicides (GBHs) may act as endocrine disruptors. We evaluated the potential estrogenic effects of a GBH formulation using the uterotrophic assay. Adult ovariectomized rats were sc injected for 3 consecutive days with: saline solution (vehicle control), 2.10(-5)g E-2/kg/day (uterotrophic dose; UE2), 2.10(-7)g E-2/kg/day (nonuterotrophic dose; NUE2), or 0.5, 5, or 50mg GBH/kg/day of the. Twenty-four hours after the last injection, the uterus was removed and weighed and processed for histopathology and mRNA extraction. Epithelial cell proliferation and height and expression of estrogen-responsive genes were evaluated (estrogen receptors, ER and ER; progesterone receptor, PR; complement 3, C3). Uterine weight and epithelial proliferation were not affected by GBH. However, the luminal epithelial cell height increased at GBH0.5. ER mRNA was downregulated by all GBH doses and E-2 groups, whereas PR and C3 mRNA were diminished by GBH0.5. GBH5-, GBH50-, and UE2-treated rats showed downregulated ER protein expression in luminal epithelial cells, while the receptor was upregulated in the stroma. GBH upregulated ER (GBH0.5-50) and PR (GBH5) expressions in glandular epithelial cells, similar effect to that of NUE2 group. These results indicate that, although the uterine weight was not affected, GBH modulates the expression of estrogen-sensitive genes. (c) 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1191-1201, 2017.
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