期刊
ANIMAL BIOTECHNOLOGY
卷 33, 期 6, 页码 1161-1169出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10495398.2021.1879824
关键词
Tudor-SN; STAT5; PRL; milk protein; cell proliferation
资金
- National Natural Science Foundation of China [31601141, 31700683]
- 'Academic Backbone' Project of Northeast Agricultural University [19XG19]
The study found that Tudor-SN positively regulates milk protein synthesis and cell proliferation by modulating STAT5 activation. This discovery may provide new insights for increasing milk production.
Tudor staphylococcal nuclease (Tudor-SN) participates in milk synthesis and cell proliferation in response to prolactin (PRL) and plays a regulatory role on mTOR phosphorylation. However, the complicated molecular mechanism of Tudor-SN regulating milk protein synthesis and cell proliferation still remains to be illustrated. In present study, we observed that the proteins level of phosphorylated Tudor-SN and phosphorylated STAT5 were simultaneously enhanced upon PRL treatment in bovine mammary epithelial cells (BMECs). Tudor-SN overexpression and knockdown experiment showed that Tudor-SN positively regulated the synthesis of milk protein, cell proliferation and the phosphorylation of STAT5, which was dependent on Tudor-SN phosphorylation. STAT5 knockdown experiment showed that Tudor-SN stimulated mTOR pathway through regulating STAT5 activation, which was required for PRL to activate the mTOR pathway. Thus, these results demonstrate the primary mechanism of Tudor-SN coordinating with STAT5 to regulate milk protein synthesis and cell proliferation under stimulation of PRL in BMECs, which may provide some new perspectives for increasing milk production.
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