Control of Triplet Blinking Using Cyclooctatetraene to Access the Dynamics of Biomolecules at the Single-Molecule Level

To explore the dynamics of biomolecules, tracing the kinetics of photo-induced chemical reactions via the triplet excited state (T-1) of probe molecules offers a timescale that is about 10(6) times wider than via the singlet excited state (S-1). Using cyclooctatetraene (COT) as a triplet energy acceptor and at the same time as a photostabilizer, the triplet-triplet energy transfer (TTET) kinetics governed by oligonucleotide (oligo) dynamics were studied at the single-molecule level by measuring fluorescence blinking. TTET kinetics measurement allowed us to access the length- and sequence-dependent dynamics of oligos and realize the single-molecule detection of a model microRNA biomarker. In sharp contrast to the singlet-singlet Forster resonance energy transfer (FRET) that occurs in the 1-10 nm range, TTET requires a Van der Waals contact. The present method is thus a complementary method to FRET and provides direct information on biomolecular dynamics on the mu s to ms timescale.

Automated summary

By measuring fluorescence blinking, the dynamics of triplet-triplet energy transfer (TTET) governed by oligonucleotide dynamics at the single-molecule level were studied. TTET kinetics measurement allowed access to length- and sequence-dependent dynamics of oligos and enabled single-molecule detection.