4.3 Article

Transport of Oligopeptide from Aqueous Phase to Span 80 Reverse Micelles in Microdroplet Array

期刊

ANALYTICAL SCIENCES
卷 37, 期 5, 页码 753-758

出版社

JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.20SCP18

关键词

Droplet microfluidics; surfactant; spontaneous emulsification

资金

  1. JST/PRESTO research program [JPMJPR15F9]
  2. Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials research program of the Network Joint Research Center for Materials and Devices
  3. Tohoku University Center for Gender Equality Promotion (TUMUG) Support Project
  4. KAKENHI [16K17503, 19H02739, 20H04691]
  5. Grants-in-Aid for Scientific Research [19H02739, 16K17503, 20H04691] Funding Source: KAKEN

向作者/读者索取更多资源

The study found that low-solubility oligopeptides require accompanying water transport to the reverse micelle phase.
The partitioning of water and tetramethylrhodamine-conjugated-10-residue oligopeptides from the aqueous phase of microdroplets into Span 80 reverse micelles was observed by utilizing microdroplet arrays. Each peptide was dissolved in phosphate buffer saline, and initially encapsulated in arrayed droplets. An organic phase containing the reverse micelles was added to the microdroplets. Here, the hydration degree of the reverse micelle was adjusted by contact of the organic phase with a 1.0 M NaCl aqueous solution or with a phosphate buffer saline before combining it with the microdroplets. For micelles treated with a 1.0 M NaCl, significant water transport from the microdroplet to the micelle was observed, and peptide with low solubility in water was transported to the reverse micelles, while those with high solubility in water were not. For micelles treated with phosphate buffer saline, the water transport was minimal, and no significant peptide transport was observed. These results suggest that the partitioning of low-solubility oligopeptides requires accompanying water transport to the reverse micelle phase.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据