期刊
ANALYTICAL CHEMISTRY
卷 93, 期 21, 页码 7739-7745出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c01252
关键词
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资金
- National Natural Science Foundation of China [22076090, 21775082]
- Shandong Province Higher Educational Program for Young Innovation Talents
- Shandong Provincial Natural Science Foundation [ZR2020ZD37]
This study presents a method for highly sensitive and simultaneous determination of multiple pesticides by target pesticide-triggered signal amplification. Compared to methods focusing on single pesticides, the proposed approach offers lower detection limits and allows for simultaneous evaluation of acetamiprid and profenofos residues in vegetables.
Credible and simultaneous determination of multiple pesticides is highly desirable for guaranteeing food safety. However, the current methods are limited to significant interference of color and fluorescence or electrode's modification and mainly focus on the analysis of a single pesticide. Herein, we proposed a novel aptamer-based homogeneous electrochemical system for highly sensitive and simultaneous analysis of multiple pesticides based on target pesticide-switched exonuclease III (Exo III)-assisted signal amplification. The recognition of hairpin probes by target pesticides impels the production of pesticide-DNA complexes, which hybridize with electroactive dye-labeled DNA to form double-stranded DNA, subsequently initiating an Exo III-assisted digestion reaction to generate abundant electroactive dye-tagged mononucleotides. In comparison with pesticide deficiency, two higher differential pulse voltammetry (DPV) currents are measured, which rely on the amount of target pesticides. Therefore, simultaneous analysis of two pesticides is realized with limits of detection of 0.0048 and 0.0089 nM, respectively, comparable or superior to those of known methods that focused on a single pesticide. Moreover, the proposed system is successfully employed to simultaneously evaluate the residual level of acetamiprid and profenofos in Brassica chinensis and thus will find more useful applications for pesticide-related food safety.
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