期刊
ANALYTICAL CHEMISTRY
卷 93, 期 18, 页码 6989-6999出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c05459
关键词
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资金
- National Research Council of Thailand [NRCT-RSA63023-01, NRCT5-RGJ63020]
- Thailand Graduate Institute of Science and Technology (TGIST)
- Department of Chemistry, Faculty of Science, Ubon Ratchathani University
- [IRN/502/2563]
This work introduces a simple method for synthesizing nitrogen-doped carbon quantum dots (N-CDs), fabricating a microfluidic paper-based analytical device (muPAD), and using them for colorimetric determination of total cholesterol (TC) in human blood. The N-CDs demonstrated high affinity towards ABTS and strong adsorption of H2O2 at the graphitic N positions. The muPAD could classify TC levels visually within 10 minutes, making it a low-cost, rapid, simple, sensitive, and selective alternative for TC detection.
This work presents a simple hydrothermal synthesis of nitrogen-doped carbon dots (N-CDs), fabrication of microfluidic paper-based analytical device (mu PAD), and their joint application for colorimetric determination of total cholesterol (TC) in human blood. The N-CDs were characterized by various techniques including transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD), and the optical and electronic properties of computational models were studied using the time-dependent density functional theory (TD-DFT). The characterization results confirmed the successful doping of nitrogen on the surface of carbon dots. The N-CDs exhibited high affinity toward 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt (ABTS) with the Michaelis-Menten constant (K-M) of 0.018 mM in a test for their peroxidase-like activity. Particularly, since hydrogen peroxide (H2O2) is the oxidative product of cholesterol in the presence of cholesterol oxidase, a sensitive and selective method of cholesterol detection was developed. Overall, the obtained results from TD-DFT confirm the strong adsorption of H2O2 on the graphitic N positions of the N-CDs. The laminated three-dimensional (3D)-mu PAD featuring a 6 mm circular detection zone was fabricated using a simple wax screen printing technique. Classification of TC according to the clinically relevant criteria (healthy, <5.2 mM; borderline, 5.2-6.2 mM; and high risk, >6.2 mM) could be determined by the naked eye within 10 min by simple comparison using a color chart. Overall, the proposed colorimetric device serves as a low-cost, rapid, simple, sensitive, and selective alternative for TC detection in whole blood samples that is friendly to unskilled end users.
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