期刊
ANALYTICAL CHEMISTRY
卷 93, 期 15, 页码 6080-6087出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c04525
关键词
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资金
- National Science Foundation of Chemistry, under CAREER award [CHE-1654274]
- Division of Molecular and Cellular Biosciences
- National Science Foundation [HRD-1547798]
This study presents a novel workflow based on complementary gas-phase separations for identifying isomeric PAHs from complex mixtures. By coupling GC, APLI, and TIMS-MS, over a hundred known unknowns were uniquely identified based on multiple characterization metrics. The workflow also demonstrated the analytical capability for untargeted screening of isomeric and isobaric compounds with additional characterization metrics not available in traditional GC-EI-MSn workflows.
In the present work, a novel workflow based on complementary gas-phase separations for the identification of isomeric PAHs from complex mixtures is described. This is the first report on the coupling of gas chromatography (GC), atmospheric pressure laser ionization (APLI), and trapped ion mobility spectrometry-mass spectrometry (TIMS-MS) for the characterization of polycyclic aromatic hydrocarbons. Over a hundred known unknowns are uniquely identified based on the molecular ion retention indices I (5%), mobility (RSD < 0.6% and R = 50-90 with S-r = 0.18 V/ms), mobility-based theoretical candidate assignment (<3%), accurate mass chemical formula assignment (<2 ppm), and electron impact fragmentation pattern and database search. The advantages of theoretical modeling of PAHs and similar compounds were evaluated using candidate structures ranked by retention indices and fragmentation pattern from GC-EI-MS data sets. Over 20 PAH isomeric and deuterated standards were utilized for the GC-APLI-TIMS-TOF MS workflow validation. Noteworthy is the analytical capability for untargeted screening of isomeric and isobaric compounds with additional characterization metrics not available in traditional GC-EI-MSn workflows.
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