期刊
ANALYTICAL CHEMISTRY
卷 93, 期 17, 页码 6673-6681出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c05336
关键词
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资金
- National Research Foundation of Korea [2017R1E1A1A01075141, 2019R1A2C3004375, 2020R1A5A1018052]
- Ministry of Trade, Industry, and Energy of Korea [20010276]
- Korea Basic Science Institute [C180310, C140110]
- National Research Council of Science & Technology (NST), Republic of Korea [C180310, C140110] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [4299990214158] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study developed a new concept of using a dual-flow SERS-based LFA sensor to improve detection sensitivity by flowing two different sizes of gold nanoparticle solutions. The sensor successfully detected thyroid-stimulating hormone less than 0.5 mu IU/mL, which cannot be measured with a SERS-based single-flow LFA sensor.
The surface-enhanced Raman scattering (SERS)based lateral flow assay (LFA) strip has been considered a highsensitivity sensor that can overcome the low sensitivity and the difficulty of quantitative analysis problems inherent in the colorimetric LFA sensor. In the SERS-based LFA strip reported so far, a liquid sample flows through the nitrocellulose membrane in a single pathway. In some cases, however, this single-flow approach still has a limitation in detection sensitivity. This study developed a conceptually new SERS-based dual-flow LFA sensor to improve the detection sensitivity in a single-flow LFA sensor. First, a 25 nm Raman reporter-labeled gold nanoparticle (AuNP) solution flowed through one way, and a 45 nm AuNP solution continuously flowed through another path. This sequential flow of two different AuNP solutions enables forming additional bright hot spots between 25 and 45 nm AuNPs in the test line, and the SERS signal is strongly enhanced. Using this SERS-based dual-flow LFA sensor, it was possible to detect thyroid-stimulating hormone less than 0.5 mu IU/mL that cannot be measured with a SERS-based single-flow LFA sensor.
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