期刊
ANALYTICAL CHEMISTRY
卷 93, 期 14, 页码 5691-5699出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c04605
关键词
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资金
- National Natural Science Foundation of China [21804073]
- Natural Science Foundation of Zhejiang Province of China [LQ18B050002]
- Open Foundation of State Key Laboratory of Chemo/Biosensing and Chemometrics [2017014]
- Natural Science Foundation of Ningbo City [2018A610315, 2019A610195]
- Ningbo University
Dual-mode imaging, combining TP fluorescence imaging and MRI, shows promise in improving accuracy for early disease diagnosis, especially in pH-related diagnosis and treatment, with great potential to enhance clinical efficacy.
Biological fluorescence imaging technologies have attracted a lot of attention and have been widely used in biomedical fields. Compared with other technologies, fluorescence imaging has a lower cost, higher sensitivity, and easier operation. However, due to the disadvantages of one-photon (OP) fluorescence imaging, such as low spatial and poor temporal resolution and poor tissue permeability depth, the application of OP fluorescence imaging has some limitations. Though two-photon (TP) fluorescence imaging can well overcome these shortcomings of OP, the single-mode imaging remains deficient. Therefore, dual-mode imaging combined with TP imaging and magnetic resonance imaging (MRI) can make up for the deficiency well, which make dual-mode imaging for the early diagnosis of diseases more accurate. Hence, a dual-mode nanoprobe TP-CQDs@MnO2 was designed for probing the fluorescence/MR dual-mode imaging strategy of intracellular H+ by using TP-CQDs (two photon-carbon quantum dots) and MnO2 nanosheets. The MnO2 nanosheets treated as fluorescence quenching agents of TP-CQDs exhibited a supersensitive response to H+, which made the fluorescence signals turn off to on for TP fluorescence imaging, in the meantime, large amounts of Mn2+ were generated for MRI. A dual-mode nanoprobe TP-CQDs@MnO2 can monitor intracellular wide pH (4.0-8.0), and the fluorescence intensity of TP-CQDs@MnO2 has recovered up to more than six times and the corresponding results of MRI were satisfactory. TP fluorescence imaging of cells and tissues showed higher detection sensitivity and deeper tissue penetration (240.0 mu m) than OP. The dual-mode imaging platform hold great promise for pH-related early diagnosis and treatment, which has great potential to improve clinical efficacy.
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