Detector-Free Photothermal Bar-Chart Microfluidic Chips (PT-Chips) for Visual Quantitative Detection of Biomarkers

The volumetric bar-chart microfluidic chips (V-Chips) driven by chemical reaction-generated gas provide a promising platform for point-of-care (POC) visual biomarker quantitation. However, multiple limitations are encountered in conventional VChips, such as costly and complex chip fabrication, complicated chip assembly, and imprecise controllability of gas production. Herein, we introduced nanomaterial-mediated photothermal effects to V-Chips, and for the first time developed a new type of V-Chip, photothermal bar-chart microfluidic chip (PT-Chip), for visual quantitative detection of biochemicals without any bulky and costly analytical instruments. Immunosensing signals were converted to visual readout signals via photothermal effects, the on-chip bar-chart movements, enabling quantitative biomarker detection on a low-cost polymer hybrid PT-Chip with on-chip scale rulers. Four different human serum samples containing a prostate-specific antigen (PSA) as a model analyte were detected simultaneously using the PT-Chip, with a limit of detection of 2.1 ng/mL, meeting clinical diagnostic requirements. Although no conventional signal detectors were used, it achieved comparable detection sensitivity to absorbance measurements with a microplate reader. The PTChip was further validated by testing human whole blood without the color interference problem, demonstrating the good analytical performance of our method even in complex matrices and thus the potential to fill the gap in current clinical diagnostics that is incapable of testing whole blood. This new PT-Chip driven by nanomaterial-mediated photothermal effects opens a new horizon of microfluidic platforms for instrument-free diagnostics at the point-of-care.

Automated summary

This study introduces nanomaterial-mediated photothermal effects to develop a new type of photothermal bar-chart microfluidic chip (PT-Chip) for visual quantitative detection of biochemicals without the need for bulky and expensive analytical instruments. The PT-Chip was able to simultaneously detect four different human serum samples, showing good analytical performance and a limit of detection of 2.1 ng/mL.