期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 413, 期 14, 页码 3725-3735出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03321-8
关键词
Lateral flow immunoassay; Inline cleanup; Carbendazim; Complex sample matrix; Rapid screening
资金
- State key R&D Program on Modernization of Traditional Chinese Medicine [2018YFC1707904]
- National Natural Science Foundation of China [81573595]
- CAMS Innovation Fund for Medical Sciences [2017-I2M-1-013]
- National Science and Technology Major Project for Significant New Drugs Development [2018ZX09721004-010]
The study introduces a sensitive colloidal gold-based lateral flow immunoassay (LFIA) method for detecting carbendazim residues in functional foods, showing good sensitivity and anti-interference ability. The established LFIA method is fast, reliable, and suitable for on-site rapid detection of carbendazim residues in complex sample matrix.
In this study, for the first time, we propose a sensitive colloidal gold-based lateral flow immunoassay (LFIA) that can be used to detect carbendazim residues in functional foods. The adoption of inline cleanup LFIA strips effectively improved background interference to reduce misjudgment of results. First, the hapten 2-(methylamino)-1H-benzo[d]imidazole-5-carboxylic acid was used to establish the carbendazim immunoassay method. Subsequently, colloidal gold-mAb preparation and LFIA detection conditions were systematically optimized. For root and fruit samples (ginseng, ginger, jujube, and Chinese wolfberry), the designed strips had a cutoff value of 8 ng/mL. For flower and seed samples (chrysanthemum, coix seed, and malt), the cutoff value was 12 ng/mL. Even in a complex matrix, the established LFIA method demonstrates satisfactory sensitivity and anti-interference ability. This method was successfully applied in detection of carbendazim residues in complex functional foods, and the assay results are consistent with those obtained via liquid chromatography-tandem mass spectrometry. In short, the proposed method is fast and sensitive and has strong anti-interference ability. Furthermore, it provides a new technical method highly relevant to the on-site rapid detection of carbendazim residues in complex sample matrix.
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