期刊
ANALYTICA CHIMICA ACTA
卷 1159, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aca.2021.338428
关键词
Electrochemical sensor; HIV DNA; Hemin/G-quadruplex; 2D DNA-Au nanowire; Polymerase chain reaction
资金
- Natural Science Foundation of Xuzhou City [KC18140]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [201910320041Z]
A multi-signal synergistically amplified electrochemical sensing method for HIV DNA was proposed, utilizing a 2D DNA-Au nanowire structure, hemin/G-quadruplex, and PCR. By combining various signal amplification processes, ultra-sensitive detection of HIV DNA was achieved, demonstrating potential for clinical diagnosis.
Multi-signal synergistically amplified electrochemical sensing of HIV DNA was proposed based on two-dimensional (2D) DNA-Au nanowire structure coupled with hemin/G-quadruplex and polymerase chain reaction (PCR). In the design, by using target HIV DNA as the template, PCR generated numbers of double-stranded DNA (dsDNA) with free single-stranded DNA (ssDNA) tails on one side and free G-quadruplex sequences on the other side. Then, the ssDNA tails of the PCR products were hybridized with the capture probe (CP) to introduce the hemin/G-quadruplex to the electrode surface as a redox-active reporter and to amplify the electrochemical signal as mimic peroxidase catalysis in the presence of H2O2. Meanwhile, (+)AuNPs were electrostatically adsorbed onto dsDNA surface for the formation of 2D DNA-Au nanowire structure, amplifying the electrochemical signal further as another mimic peroxidase and electric conductor together. By effectively combining these signal amplification processes, ultra-sensitive HIV DNA detection was achieved with a detection limit of 1.3 aM, indicating that it has potential application in clinical diagnosis. (C) 2021 Elsevier B.V. All rights reserved.
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