期刊
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
卷 65, 期 1, 页码 81-91出版社
AMER THORACIC SOC
DOI: 10.1165/rcmb.2021-0004OC
关键词
bronchopulmonary dysplasia; lung development; capillary development; stereology; serial block-face scanning electron microscopy
资金
- Deutsche Forschungsgemeinschaft [MU 3118/8-1, MO 1789/4-1]
The study conducted on neonatal mice exposed to different oxygen concentrations revealed that disrupted vascular development may lead to impaired alveolarization, supporting the vascular hypothesis of BPD. Alveolar capillary network in hyperoxia-exposed mice showed rarefaction and altered geometry, with effects on alveolar epithelium and capillary endothelium appearing at different time points. These findings provide valuable insights into the pathogenesis of BPD.
Bronchopulmonary dysplasia (BPD), the most common sequela of preterm birth, is a severe disorder of the lung that is often associated with long-lasting morbidity. A hallmark of BPD is the disruption of alveolarization, whose pathogenesis is incompletely understood. Here, we tested the vascular hypothesis that disordered vascular development precedes the decreased alveolarization associated with BPD. Neonatal mouse pups were exposed to 7, 14, or 21 days of normoxia (21% O-2) or hyperoxia (85% O-2) with n58-11 for each group. The right lungs were fixed by vascular perfusion and investigated by design-based stereology or three-dimensional reconstruction of data sets obtained by serial block-face scanning EM. The alveolar capillary network of hyperoxia-exposed mice was characterized by rarefaction, partially altered geometry, and widening of capillary segments as shown by three-dimensional reconstruction. Stereology revealed that the development of alveolar epithelium and capillary endothelium was decreased in hyperoxia-exposed mice; however, the time course of these effects was different. That the surface area of the alveolar epithelium was smaller in hyperoxia-exposed mice first became evident at Day 14. In contrast, the surface area of the endothelium was reduced in hyperoxia-exposed mouse pups at Day 7. The thickness of the air-blood barrier decreased during postnatal development in normoxic mice, whereas it increased in hyperoxic mice. The endothelium and the septal connective tissue made appreciable contributions to the thickened septa. In conclusion, the present study provides clear support for the idea that the stunted alveolarization follows the disordered microvascular development, thus supporting the vascular hypothesis of BPD.
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