Direct assessment of individual skin barrier components by electrical impedance spectroscopy

Background Expression of the tight junction proteins Cldn1 and 4 is altered in skin diseases such as atopic dermatitis, and Cldn1 deficiency affects skin barrier formation. Impedance spectroscopy (IS) has been proven to allow detection of alterations in the skin barrier but is currently unable to separate effects on viable epidermis (VE) and stratum corneum (SC). Methods Effects of siRNA-mediated Cldn1 and 4 knockdown in reconstructed human epidermis (RHE) on VE and SC barrier function were investigated with Ussing chamber-based IS. Barrier components were sequentially altered, employing iron oxide nanoparticles and EGTA, to identify their contribution to the impedance spectrum. Resistance changes due to apically applied hyperosmolar electrolyte were used to identify barrier defects non-invasively. Results IS of RHE yielded two relaxation frequencies, representing the barrier properties of the SC (similar to 1000 Hz) and VE (similar to 100 Hz). As proof of concept, it was shown that the Cldn1 knockdown-induced resistance drop arises from the impairment of both SC and VE, indicated by a shift of both relaxation frequencies. Hyperosmolar electrolyte penetration allowed non-invasive detection of Cldn1 knockdown via time-dependent frequency shifts. The absence of Cldn4 knockdown-induced changes revealed the weaknesses of transepithelial electrical resistance analysis. Conclusion In conclusion, the present technique allows to separately measure the barrier properties of SC and VE and further evaluate the Cldn1 and 4 knockdown impact on the skin barrier. As the measurement with agarose-embedded electrolyte allowed non-invasive identification of the Cldn1 knockdown, this opens the way to detailed in vivo skin barrier assessment.

Automated summary

This study utilized impedance spectroscopy to separate the barrier properties of the stratum corneum (SC) and viable epidermis (VE), and evaluate the impact of Cldn1 and 4 knockdown on the skin barrier. The results showed that the resistance drop induced by Cldn1 knockdown arises from impairment of both SC and VE, and non-invasive detection of Cldn1 knockdown was achieved through time-dependent frequency shifts with hyperosmolar electrolyte penetration.