Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma

Annexin A4 (ANXA4) is a Ca2+- and phospholipid-binding protein that belongs to the annexin family, which is involved in the development of multiple tumour types via NF-kappa B signalling. In this study, we verified the high expression and membrane-cytoplasm translocation of ANXA4 in colorectal carcinoma (CRC). Calcium/calmodulindependent protein kinase II gamma (CAMK2 gamma) was found to be important for high ANXA4 expression in CRC, whereas carbonic anhydrase (CA1) promoted ANXA4 aggregation in the cell membrane. An increased Ca2+ concentration attenuated the small ubiquitin-like modifier (SUMO) modification of cytoplasmic ANXA4 and ANXA4 stabilization, and relatively high expression of ANXA4 promoted CRC tumorigenesis and epithelial-mesenchymal transition (EMT).

Automated summary

Annexin A4 (ANXA4) is highly expressed and translocated to the membrane and cytoplasm in colorectal carcinoma (CRC). CAMK2 gamma is important for high ANXA4 expression, while CA1 promotes ANXA4 aggregation on the cell membrane. Increased Ca2+ concentration attenuates SUMO modification of cytoplasmic ANXA4, leading to ANXA4 stabilization and promotion of CRC tumorigenesis and EMT.