期刊
ACTA BIOMATERIALIA
卷 124, 期 -, 页码 191-204出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2021.01.029
关键词
Hydrogel; Photocurable nanocomposite; Nanofiber; Growth factor; Sustained release; Skin repair
资金
- Sharif University of Technology [QA970816]
- Iran National Science Foundation (INSF) [95S48740]
The engineered bilayer scaffolds, composed of gelatin nanofibers and composite hydrogels, show promising potential in accelerating wound healing process. The controlled delivery of growth factors on the bilayer scaffolds enhances wound closure and tissue regeneration. The unique structure of the scaffolds mimics the extracellular matrix, providing a platform for tailored growth factor release and improved adhesion to native tissue.
Wound healing is a complex process based on the coordinated signaling molecules and dynamic interactions between the engineered scaffold and newly formed tissue. So far, most of the engineered scaffolds used for the healing of full-thickness skin wounds do not mimic the natural extracellular matrix (ECM) complexity and therefore are not able to provide an appropriate niche for endogenous tissue regeneration [1] . To address this gap and to accelerate the wound healing process, we present biomimetic bilayer scaffolds compositing of gelatin nanofibers (GFS) and photocrosslinkable composite hydrogels loaded with epidermal growth factors (EGF). The nanofibers operate as the dermis layer, and EGF-loaded composite hydrogels acted as the epidermis matrix for the full-thickness wound healing application. The hydrogels are composed of gelatin metacryloyl (GelMA) modified with silicate nanoplatelets (Laponite). To overcome the challenges of transdermal delivery of EGF, including short half-life and lack of efficient formulation precise, controlled delivery was attained by immobilization of EGF on Laponite. It is shown that the addition of 1wt% silicate nanoplatelet increases the compressive modulus of the hydrogels by 170%. In vitro wound closure analysis also demonstrated improved adhesion of the scaffolds to the native tissue by 3.5 folds. Moreover, the tunable hemostatic ability of the scaffolds due to the negatively charged nanoplatelets is shown. In an established excisional full-thickness wound model, an enhanced wound closure (up to 93.1 ? 1.5%) after 14 days relative to controls (GFS and saline-treated groups) is demonstrated. The engineered adhesive and hemostatic scaffolds with sustained release of the growth factors have the potential to stimulate complete skin regeneration for full-thickness wound healing. Statement of Significance A combination of highly porous nanocomposite hydrogel and nanofibrous structure of electrospun membrane provides a unique platform to mimic the structure of the extracellular matrix. We have introduced bilayer scaffolds with different structures as a functional platform for the controlled release of growth factors for enhanced full-thickness cutaneous wound healing. The mechanical properties of the bilayer scaffold are shown to be close to the native skin tissue. In vitro assays affirm hemostatic and tissue adhesive properties by the bilayer scaffold. Histological analysis also determines the superior wound healing performance of the bilayer scaffold in terms of wound closure, granulation tissue, skin appendage, and collagen metabolism.
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