Advances in 3D cell culture for liver preclinical studies

The 3D cell culture model is an indispensable tool in the study of liver biology in the field of health and disease and the development of clinically relevant products for liver therapies. The 3D culture model captures critical factors of the microenvironmental niche required by hepatocytes for exhibiting optimal phenotypes, thus enabling the pursuit of a range of preclinical studies that are not entirely feasible in conventional 2D cell models. In this review, we highlight the major attributes associated with and the components needed for the development of a functional 3D liver culture model for a range of applications.

Automated summary

The 3D cell culture model is essential in studying liver biology and developing clinically relevant products for liver therapies, capturing critical microenvironmental factors for optimal phenotypes of hepatocytes. This model enables a range of preclinical studies that are not feasible in conventional 2D cell models, highlighting its major attributes and components needed for various applications.