4.8 Article

Single Cell-like Systems Reveal Active Unidirectional and Light-Controlled Transport by Nanomachineries

期刊

ACS NANO
卷 15, 期 4, 页码 6747-6755

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acsnano.0c10139

关键词

ABC transporter; giant liposomes; membrane transport; single-liposome analysis; synthetic biology

资金

  1. German Research Foundation [SFB 807, TA 157/12-1, GRK 1986]
  2. LOEWE program
  3. European Research Council [789121]
  4. European Research Council (ERC) [789121] Funding Source: European Research Council (ERC)

向作者/读者索取更多资源

Cellular life relies on transport and communication across membranes, making membrane proteins prime drug targets. Researchers have successfully reconstituted membrane assemblies using giant liposomes, demonstrating the unidirectional transport of substrates by nanomachineries. This approach enables the construction of delicate artificial cell-like systems for real-time tracking of substrate transport.
Cellular life depends on transport and communication across membranes, which is emphasized by the fact that membrane proteins are prime drug targets. The cell-like environment of membrane proteins has gained increasing attention based on its important role in function and regulation. As a versatile scaffold for bottom-up synthetic biology and nanoscience, giant liposomes represent minimalistic models of living cells. Nevertheless, the incorporation of fragile multiprotein membrane complexes still remains a major challenge. Here, we report on an approach for the functional reconstitution of membrane assemblies exemplified by human and bacterial ATP-binding cassette (ABC) transporters. We reveal that these nanomachineries transport substrates unidirectionally against a steep concentration gradient. Active substrate transport can be spatiotemporally resolved in single cell-like compartments by light, enabling real-time tracking of substrate export and import in individual liposomes. This approach will help to construct delicate artificial cell-like systems.

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