4.6 Article

Binding Mechanisms of Amyloid-like Peptides to Lipid Bilayers and Effects of Divalent Cations

期刊

ACS CHEMICAL NEUROSCIENCE
卷 12, 期 11, 页码 2027-2035

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acschemneuro.1c00140

关键词

Amyloid; lipid membrane; calcium; amyloid-membrane binding; calcium-membrane binding; Alzheimer's disease

资金

  1. National Science Foundation [ACI-1548562, CHE-1904364, CHE-1904528]

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The study found that electrostatic and hydrophobic interactions play important roles in the peptide-bilayer binding process. Peptides are strongly attracted to anionic lipid bilayers due to electrostatic interactions between positive residues and negative phosphate moieties. However, the presence of calcium ions significantly reduces the binding of peptides to the bilayer.
In several neurodegenerative diseases, cell toxicity can emerge from damage produced by amyloid aggregates to lipid membranes. The details accounting for this damage are poorly understood including how individual amyloid peptides interact with phospholipid membranes before aggregation. Here, we use all-atom molecular dynamics simulations to investigate the molecular mechanisms accounting for amyloid-membrane interactions and the role played by calcium ions in this interaction. Model peptides known to self-assemble into amyloid fibrils and bilayer made from zwitterionic and anionic lipids are used in this study. We find that both electrostatic and hydrophobic interactions contribute to peptide-bilayer binding. In particular, the attraction of peptides to lipid bilayers is dominated by electrostatic interactions between positive residues and negative phosphate moieties of lipid head groups. This attraction is stronger for anionic bilayers than for zwitterionic ones. Hydrophobicity drives the burial of nonpolar residues into the interior of the bilayer producing strong binding in our simulations. Moreover, we observe that the attraction of peptides to the bilayer is significantly reduced in the presence of calcium ions. This is due to the binding of calcium ions to negative phosphate moieties of lipid head groups, which leaves phospholipid bilayers with a net positive charge. Strong binding of the peptide to the membrane occurs less frequently in the presence of calcium ions and involves the formation of a Ca2+ bridge.

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