Electrochemical Trans-Channel Assay for Efficient Evaluation of Tumor Cell Invasiveness

Efficiently assessing the invasive capability of tumor cells is critical both for the research and treatment of cancer. Here, we report a novel method called the electrochemical trans-channel assay for efficient evaluation of tumor cell invasiveness. A bioinspired extracellular matrix degradation model (EDM) has been first fabricated on a porous anodic alumina (PAA) membrane to construct the electrochemical apparatus. Upon contacting the invasive tumor cells, invasive capability can be sensitively evaluated by the degree of EDM impairment, which is recorded by the electrochemical trans-channel ionic currents in a label-free manner. Compared to the most commonly used trans-well migration method, this assay can be accomplished in an efficient way that is significantly faster (20 min) and more convenient. Besides, quantitation can also be realized for monitoring the invasion process, which cannot be achieved by other currently used methods. Our proposed electrochemical trans-channel assay method has shown a synergistic effect for the evaluation of tumor cell invasiveness, providing a promising method for clinical assessment or prognostic applications of tumor metastasis.

Automated summary

The study introduces a novel method called the electrochemical trans-channel assay for efficiently evaluating tumor cell invasiveness. By using a bioinspired extracellular matrix degradation model to construct the electrochemical apparatus, the invasive capability of tumor cells can be sensitively evaluated, providing a faster and more convenient alternative to traditional migration methods. This assay also allows for quantification of the invasion process, offering a promising approach for clinical assessment or prognostic applications of tumor metastasis.