期刊
ACS APPLIED MATERIALS & INTERFACES
卷 13, 期 14, 页码 16828-16836出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c00215
关键词
photoelectrochemical; zinc phthalocyanine; alginate micelle; CdS; Hg2+
资金
- Natural Science Foundation of Shandong Province of China [ZR2019MB051]
- Shandong Provincial Key Research and Development Program [2018GGX102001]
- Foundation of Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science [SATM201701]
A simple and selective photoelectrochemical biosensor was developed for Hg2+ detection using zinc phthalocyanine dye-sensitized CdS and alginate as a carrier and binder. The biosensor exhibited a wide linear range and low detection limit, making it a promising platform for detecting other biomolecules. Detection of Hg2+ was achieved through specific binding with thymine-rich probe DNA, resulting in a decrease in photocurrent.
A simple and selective photoelectrochemical (PEC) biosensor was constructed for Hg2+ detection based on zinc phthalocyanine (ZnPc) dye-sensitized CdS using alginate not only as a carrier but also as a binder. First, CdS as a photoactive material was in situ modified on the electrode surface using a rapid and simple electrodeposition to obtain an initial photocurrent signal. Second, ZnPc was loaded in the amphiphilic alginate micelle and then was coated onto the CdS film surface via alginate as the binder. The photocurrent was subsequently enhanced due to the favorable dye sensitization effect of ZnPc to CdS. Finally, the thymine-rich probe DNA was immobilized on the modified ITO surface via coupling reaction between the carbonyl groups of the amphiphilic polymer and the amino groups of the probe DNA. In the presence of Hg2+, the thymine-Hg2+-thymine (T-Hg2+-T) structure was formed due to the specific bond of Hg2+ with thymine, resulting in the decrease of photocurrent due to the increase of steric hindrance on the modified electrode surface. The proposed PEC biosensor for Hg2+ detection possessed a wide linear range from 10 pM to 1.0 mu M with a detection limit of 5.7 pM. This biosensor provides a promising platform for detecting other biomolecules of interest.
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